Harris J R, Markl J
Institute of Zoology, University of Mainz, Germany.
Eur Urol. 2000;37 Suppl 3:24-33. doi: 10.1159/000052389.
In this short review we present a survey of the available biochemical and electron microscopic data on keyhole limpet hemocyanin (KLH).
The biosynthesis of KLH and its biological role are discussed and the purification of the two isoforms of KLH (KLH1 and KLH2) presented in some detail. The determination of the molecular mass of KLH, its functional unit structure, carbohydrate content, immunological analysis and aspects of the molecular biology of KLH are all dealt with. Transmission electron microscopy (TEM) and crossed immunoelectrophoresis have played a significant part in the understanding of KLH structure. We present a summary of TEM studies on the native oligomers of KLH, the experimental manipulation of the different oligomeric states, immunological analysis and subunit reassociation.
This fundamental structural information provides the scientific background upon which the understanding of the in vivo immunostimulatory function of KLH can be based.
在本简短综述中，我们对现有关于钥孔戚血蓝蛋白（KLH）的生化和电子显微镜数据进行了调查。
讨论了KLH的生物合成及其生物学作用，并详细介绍了KLH两种同工型（KLH1和KLH2）的纯化过程。还涉及了KLH分子量的测定、其功能单元结构、碳水化合物含量、免疫分析以及KLH分子生物学的各个方面。透射电子显微镜（TEM）和交叉免疫电泳在理解KLH结构方面发挥了重要作用。我们总结了关于KLH天然寡聚体的TEM研究、不同寡聚状态的实验操作、免疫分析和亚基重新缔合。
这一基本结构信息为理解KLH的体内免疫刺激功能提供了科学背景。
