Markl J, Lieb B, Gebauer W, Altenhein B, Meissner U, Harris J R
Institute of Zoology, University of Mainz, Germany.
J Cancer Res Clin Oncol. 2001 Oct;127 Suppl 2(Suppl 2):R3-9. doi: 10.1007/BF01470992.
Keyhole limpet hemocyanin (KLH) is a well-established immune stimulant and hapten carrier, and Haliotis tuberculata hemocyanin (HtH) is a related product. Biologically, KLH and HtH are blue copper proteins which serve as oxygen carriers in the blood of the keyhole limpet Megathura crenulata and the abalone H. tuberculata, respectively, two marine gastropods. Both hemocyanins occur as two distinct isoforms, termed KLH1 KLH2, HtH1, and HtH2. Each of these molecules is based on a very large polypeptide chain, the subunit (molecular mass ca 400 kDa), which is folded into a series of eight globular functional units (molecular mass ca 50 kDa each). Twenty copies of this subunit form a cylindrical quaternary structure (molecular mass ca 8 MDa). This article reviews the recent data on the biosynthesis, quaternary structure, subunit architecture, amino acid sequence, gene structure, and recombinant production of KLH and HtH.
钥孔血蓝蛋白（KLH）是一种成熟的免疫刺激剂和半抗原载体，而皱纹盘鲍血蓝蛋白（HtH）是一种相关产品。从生物学角度来看，KLH和HtH是蓝色铜蛋白，分别在两种海洋腹足纲动物——钥孔帽贝巨缘孔帽贝和皱纹盘鲍的血液中充当氧载体。两种血蓝蛋白均以两种不同的同工型存在，分别称为KLH1、KLH2、HtH1和HtH2。这些分子中的每一个都基于一条非常大的多肽链，即亚基（分子量约400 kDa），该亚基折叠成一系列八个球状功能单元（每个功能单元分子量约50 kDa）。该亚基的二十个拷贝形成一个圆柱形四级结构（分子量约8 MDa）。本文综述了关于KLH和HtH的生物合成、四级结构、亚基结构、氨基酸序列、基因结构和重组生产的最新数据。
