Yang Y, Ding B, Baulcombe D C, Verchot J
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74078, USA.
Mol Plant Microbe Interact. 2000 Jun;13(6):599-605. doi: 10.1094/MPMI.2000.13.6.599.
The 25K, 12K, and 8K proteins and coat protein (CP) of Potato virus X (PVX) are required for virus cell-to-cell movement. In this study, experiments were conducted to determine whether the PVX 25K protein moves cell to cell and to explore potential interactions between the PVX 25K, 12K, and 8K proteins and CP. The PVX 25K gene was fused to the green fluorescent protein (GFP) gene and inserted into plasmids adjacent to the cauliflower mosaic virus 35S promoter. These plasmids were introduced by biolistic bombardment to transgenic tobacco expressing the PVX 12K, 8K, and CP genes. The GFP:25K fused proteins moved cell to cell on nontransgenic tobacco and tobacco expressing either the 12K or 8K proteins. However, the GFP:25K proteins did not move on transgenic tobacco expressing the combined 12K/8K genes or the CP gene. Thus, movement of the PVX 25K protein through plasmodesmata may be regulated by interactions with other PVX proteins.
马铃薯X病毒（PVX）的25K、12K和8K蛋白以及外壳蛋白（CP）是病毒细胞间移动所必需的。在本研究中，进行了实验以确定PVX 25K蛋白是否能在细胞间移动，并探索PVX 25K、12K和8K蛋白与CP之间的潜在相互作用。将PVX 25K基因与绿色荧光蛋白（GFP）基因融合，并插入到与花椰菜花叶病毒35S启动子相邻位置的质粒中。通过生物弹轰击将这些质粒导入表达PVX 12K、8K和CP基因的转基因烟草中。GFP:25K融合蛋白能在非转基因烟草以及表达12K或8K蛋白的烟草中进行细胞间移动。然而，GFP:25K蛋白在表达12K/8K组合基因或CP基因的转基因烟草中不能移动。因此，PVX 25K蛋白通过胞间连丝的移动可能受到与其他PVX蛋白相互作用的调控。
