Kimoto S, Yamamoto Y, Yamagami K, Ishikawa Y, Kume M, Yamamoto H, Ozaki N, Yamaoka Y
Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Japan.
Int J Hyperthermia. 2000 May-Jun;16(3):247-61. doi: 10.1080/026567300285268.
Heat shock pretreatment induces heat shock protein (HSP)72 strongly in rat livers and provides the tolerance against subsequent ischemia-reperfusion injury. In this study, the effects of repeated heat shock pretreatment on the production of HSP72 in rat livers and on subsequent ischemic tolerance were investigated.
Rats pretreated with repeated heat shock were compared with those that received a single heat shock pretreatment. The production of HSP72 was analysed using Western-blotting and densitometer. At 48 h after heat shock pretreatment, all rats were subjected to warm liver ischemia for 30 or 45 min and then reperfused. Survival rate of the animals and liver functions during reperfusion were analysed.
The production of HSP72 increased in the repeated heat shock group more than in the single heat shock group. Although there were no significant differences in animal survival or in liver functions after a 30-min ischemia between the single heat shock group and the repeated heat shock group, animal survival and liver functions after a 45-min ischemia were significantly better in the repeated heat shock group.
In rats, repetition of heat shock pretreatment augmented the production of HSP72 in liver tissue and protected the liver from ischemia-reperfusion injury.
热休克预处理可在大鼠肝脏中强烈诱导热休克蛋白（HSP）72的产生，并提供对随后缺血再灌注损伤的耐受性。在本研究中，探讨了重复热休克预处理对大鼠肝脏中HSP72产生及随后缺血耐受性的影响。
将接受重复热休克预处理的大鼠与接受单次热休克预处理的大鼠进行比较。使用蛋白质免疫印迹法和密度计分析HSP72的产生。在热休克预处理后48小时，所有大鼠进行30或45分钟的温性肝缺血，然后再灌注。分析动物的存活率和再灌注期间的肝功能。
重复热休克组中HSP72的产生比单次热休克组增加更多。虽然单次热休克组和重复热休克组在30分钟缺血后动物存活率或肝功能方面没有显著差异，但重复热休克组在45分钟缺血后的动物存活率和肝功能明显更好。
在大鼠中，重复热休克预处理可增强肝脏组织中HSP72的产生，并保护肝脏免受缺血再灌注损伤。
