Choi S I, Choi S H, Kim S T, Lim K H, Lim C H, Gong G Y, Kim H Y, Weinmann H J, Lim T H
Department of Diagnostic Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Radiology. 2000 Jun;215(3):863-8. doi: 10.1148/radiology.215.3.r00jn01863.
To investigate the capability of a necrosis-avid magnetic resonance (MR) contrast agent, bis-gadolinium mesoporphyrins, for assessment of irreversibly damaged myocardium and to evaluate the time course of signal enhancement in the reperfused myocardium.
Nine cats were subjected to 90 minutes of occlusion of the left anterior descending coronary artery followed by 90 minutes of reperfusion. Contrast material-enhanced T1-weighted spin-echo images were obtained for 12 hours in five cats and 6 hours in four cats. Pathologic examinations of the resected specimens were performed with 2'3'5-triphenyl tetrazolium chloride (TTC) histochemical staining and electron microscopy. The size of enhanced area on MR images was compared with that of irreversibly damaged myocardium with TTC staining. The time course of signal enhancement was evaluated.
The size of enhanced area on MR images was well correlated with that of irreversibly damaged myocardium with TTC staining. Maximum enhancement occurred 1-3 hours after administration of the contrast material, with mean enhancement of 171% that of normal myocardium. Electron microscopic examinations showed severe myocardial damage in the irreversibly damaged myocardium but only mild edematous changes in the reversibly damaged myocardium.
MR images enhanced with bis-gadolinium mesoporphyrins provide accurate sizing of irreversibly damaged myocardium with a strong and persistent signal enhancement in the reperfused myocardium.
研究坏死亲和性磁共振（MR）造影剂双钆中卟啉评估不可逆损伤心肌的能力，并评估再灌注心肌信号增强的时间进程。
对9只猫进行左前降支冠状动脉闭塞90分钟，随后再灌注90分钟。5只猫在12小时内、4只猫在6小时内获取对比剂增强的T1加权自旋回波图像。对切除标本进行2,3,5-三苯基氯化四氮唑（TTC）组织化学染色和电子显微镜检查。将MR图像上增强区域的大小与TTC染色显示的不可逆损伤心肌的大小进行比较。评估信号增强的时间进程。
MR图像上增强区域的大小与TTC染色显示的不可逆损伤心肌的大小密切相关。注射对比剂后1 - 3小时出现最大增强，平均增强为正常心肌的171%。电子显微镜检查显示不可逆损伤心肌有严重心肌损伤，而可逆损伤心肌仅有轻度水肿改变。
双钆中卟啉增强的MR图像能准确测量不可逆损伤心肌的大小，且再灌注心肌有强烈且持续的信号增强。
