Tafalla C, Novoa B
Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello, 6, 36208, Vigo, Spain.
Dev Comp Immunol. 2000 Sep-Oct;24(6-7):623-31. doi: 10.1016/s0145-305x(99)00087-7.
The effect of different cytokines and bacterial lipopolysaccharide (LPS) on turbot (Scophthalmus maximus) macrophage nitric oxide (NO) production has been studied. We have found two different responses concerning NO production in response to LPS. We have studied 43 turbot and only macrophage cultures derived from 30.2% of these turbot were significantly stimulated by LPS. The macrophage populations that did not respond to LPS, showed a constitutive production that was significantly reversed by NO inhibitors like N(G)-methyl-L-arginine (L-NMMA) and N-omega-nitro-L-arginine (L-NAME), and was dependent on intracellular calcium concentration. We studied the effect of other stimuli combined with LPS on the NO production of these otherwise non-responsive macrophages. LPS combined with turbot macrophage activating factor (MAF) containing supernatants, was capable of significantly stimulating some of these macrophage populations. The same response was observed when LPS was combined with turbot IFN-alphabeta-like substances. When LPS was combined with human recombinant tumor necrosis factor alpha (hrTNF-alpha), the NO production was significantly induced in all macrophage populations studied.
研究了不同细胞因子和细菌脂多糖（LPS）对大菱鲆（Scophthalmus maximus）巨噬细胞一氧化氮（NO）产生的影响。我们发现，在对LPS产生反应时，NO的产生存在两种不同的反应。我们研究了43条大菱鲆，只有来自其中30.2%大菱鲆的巨噬细胞培养物受到LPS的显著刺激。对LPS无反应的巨噬细胞群体表现出组成型产生，这种产生被N（G）-甲基-L-精氨酸（L-NMMA）和N-ω-硝基-L-精氨酸（L-NAME）等NO抑制剂显著逆转，并且依赖于细胞内钙浓度。我们研究了其他刺激物与LPS联合对这些原本无反应的巨噬细胞NO产生的影响。LPS与含有大菱鲆巨噬细胞激活因子（MAF）的上清液联合，能够显著刺激其中一些巨噬细胞群体。当LPS与大菱鲆IFN-αβ样物质联合时，观察到相同的反应。当LPS与人重组肿瘤坏死因子α（hrTNF-α）联合时，在所研究的所有巨噬细胞群体中，NO的产生均被显著诱导。
