6,6'-二硫代烟酸与经硫醇化合物预处理的大鼠胸腺细胞的相互作用。
Interaction of 6,6'-dithiodinicotinic acid with rat thymocytes pretreated with thiol compounds.
作者信息
Krzystyniak K, Roszkowski-Sliz W, Ryzewski J
出版信息
Arch Immunol Ther Exp (Warsz). 1976;24(1):109-14.
The number of sulfhydryl groups in the membranes of rat thymocytes were determined, expressed in terms of concentration of 6-mercaptonicotinic acid produced by reduction of 6,6'-dithiodinicotinic acid. The influence of incubation of thymocytes in solutions of L-cysteine, reduced glutathione and dithiothreitol on the number of -SH groups was studied. About twofold higher degree of reduction of 6-6'dithiodinicotinic acid was observed in the reaction with thymocytes incubated previously in 2 mM solution of L-cysteine in comparison with controls. Incubation of thymocytes with dithiothreitol and gluthathione had no effect on the number of -SH groups.
测定了大鼠胸腺细胞膜中巯基的数量，以6,6'-二硫代烟酸还原产生的6-巯基烟酸浓度表示。研究了胸腺细胞在L-半胱氨酸、还原型谷胱甘肽和二硫苏糖醇溶液中孵育对-SH基团数量的影响。与对照组相比，在2 mM L-半胱氨酸溶液中预先孵育的胸腺细胞反应中，观察到6,6'-二硫代烟酸的还原程度高出约两倍。胸腺细胞与二硫苏糖醇和谷胱甘肽孵育对-SH基团数量没有影响。
Zotero 插件