Noguchi S, Bukowski R, Deodhar S, Hewlett J S
Cancer. 1976 May;37(5):2247-54. doi: 10.1002/1097-0142(197605)37:5<2247::aid-cncr2820370514>3.0.co;2-c.
T- and B-cell markers of lymphocytes in peripheral blood, involved node and spleen, PHA response of peripheral blood lymphocytes, serum immunoglobulin levels, and skin test reactivity to six common antigens were studied in 16 cases of untreated non-Hodgkin's lymphoma. Impaired response of peripheral lymphocytes to PHA was observed in 13 of 16 cases, regardless of the proportion of T lymphocytes. Of 12 cases in which skin tests were done, two were positive and had a normal PHA response, seven cases were positive in spite of low PHA response, and three were negative with low PHA response. In the lymph nodes from involved areas two cases showed monoclonal increase of B-cells, five showed "null" cell increase, and the remaining nine showed no increase or decrease of subpopulation of lymphocytes. No correlation with surface marker of lymphocytes to histologic classification was seen. From the above observations it was concluded: 1) a low PHA response in non-Hodgkin's lymphoma was not due to the decreased population of T-cells; 2) a low PHA response may not necessarily indicate impaired delayed hypersensitivity; and 3) non-Hodgkin's lymphoma can be classified in the following ways--B-cell proliferative type, "null" cell increase type, and normal T/B proportion type.
对16例未经治疗的非霍奇金淋巴瘤患者外周血、受累淋巴结和脾脏中的淋巴细胞T细胞和B细胞标志物、外周血淋巴细胞的PHA反应、血清免疫球蛋白水平以及对六种常见抗原的皮肤试验反应性进行了研究。16例中有13例观察到外周淋巴细胞对PHA的反应受损，与T淋巴细胞比例无关。在进行皮肤试验的12例患者中，2例呈阳性且PHA反应正常，7例尽管PHA反应低但呈阳性，3例呈阴性且PHA反应低。在受累区域的淋巴结中，2例显示B细胞单克隆增加，5例显示“无标记”细胞增加，其余9例淋巴细胞亚群无增加或减少。未发现淋巴细胞表面标志物与组织学分类之间的相关性。根据上述观察结果得出以下结论：1）非霍奇金淋巴瘤中PHA反应低并非由于T细胞数量减少；2）PHA反应低不一定表明迟发型超敏反应受损；3）非霍奇金淋巴瘤可按以下方式分类——B细胞增殖型、“无标记”细胞增加型和T/B比例正常型。
