Yurchenko V, Kolesnikov A A, Lukes J
Department of Molecular Biology, Faculty of Biology, Moscow State University, Russia.
Folia Parasitol (Praha). 2000;47(1):1-5. doi: 10.14411/fp.2000.001.
Phylogenetic relationships within the suborder Trypanosomatina were inferred from the kinetoplast DNA minicircle conserved region sequences. Trees built using distance-matrix (Neighbor-Joining) and maximum parsimony methods showed that the minicircle conserved regions (CRs) provide a sensitive and specific molecular marker suitable for phylogenetic analyses of subspecies and strains of trypanosomatid flagellates, as testified by the subdivision of the genus Leishmania into the subgenera Leishmania. Viannia and Sauroleishmania. However, since Phytomonas and monogenetic parasites of insects represent the earliest diverging groups, the CRs do not seem to be useful for inference of relationships among major lineages of the order Kinetoplastida.
基于动质体DNA小环保守区序列推断了锥虫亚目内的系统发育关系。使用距离矩阵（邻接法）和最大简约法构建的树状图表明，小环保守区（CRs）提供了一个灵敏且特异的分子标记，适用于对锥虫鞭毛虫的亚种和菌株进行系统发育分析，利什曼原虫属分为利什曼原虫亚属、维扬亚属和沙罗利什曼原虫亚属就证明了这一点。然而，由于植滴虫和昆虫的单殖寄生虫代表了最早分化的类群，CRs似乎对于推断动基体目主要谱系之间的关系并无用处。
