Valdezate S, Echeita A, Díez R, Usera M A
Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid, Spain.
Eur J Clin Microbiol Infect Dis. 2000 Apr;19(4):275-81. doi: 10.1007/s100960050475.
Over the last 4 years Salmonella hadar has increasingly been isolated in Europe in conjunction with food-borne gastroenteritis. The aim of this study was to evaluate epidemiological methods (phage typing, antimicrobial susceptibility testing, DNA plasmid analysis, ribotyping and pulsed-field gel electrophoresis analysis) for characterising Salmonella hadar isolates. The 100% phage typeability of isolates and the high discriminatory index of 0.8856 suggest that phage typing is the method of choice. In order to obtain subdivisions of the most frequent Salmonella hadar phage types, a combination of molecular methods, such as ribotyping performed with Bg/I and EcoRI or pulsed-field gel electrophoresis using XbaI and XhoI, would be desirable as the usefulness of each technique varies with the phage type being analysed. Of note was the high (86%) rate of resistance to tetracycline and nalidixic acid but full susceptibility to ciprofloxacin in the strains studied.
在过去4年里，哈达尔沙门氏菌在欧洲与食源性肠胃炎相关联的情况下越来越多地被分离出来。本研究的目的是评估用于鉴定哈达尔沙门氏菌分离株的流行病学方法（噬菌体分型、抗菌药敏试验、DNA质粒分析、核糖体分型和脉冲场凝胶电泳分析）。分离株100%可进行噬菌体分型以及0.8856的高鉴别指数表明噬菌体分型是首选方法。为了获得最常见的哈达尔沙门氏菌噬菌体分型的细分，将分子方法结合起来是可取的，例如用Bg/I和EcoRI进行核糖体分型，或使用XbaI和XhoI进行脉冲场凝胶电泳，因为每种技术的有用性随所分析的噬菌体分型而变化。值得注意的是，在所研究的菌株中，对四环素和萘啶酸的耐药率很高（86%），但对环丙沙星完全敏感。
