Ling M L, Wang G C
Department of Pathology, Singapore General Hospital, Singapore.
J Infect. 2001 Oct;43(3):169-72. doi: 10.1053/jinf.2001.0877.
Salmonella enteritidis is the most common non-typhoidal Salmonella species isolated in Singapore causing gastroenteritis and occasional bacteremia with secondary complications. The number of S. enteritidis isolates rose in 1993 and since then, it was the commonest Salmonella sp. isolated. In 1997, a total of 139 S. enteritidis was isolated and this comprised 19.2% of all non-typhoidal Salmonella sp. isolated.
We studied the antimicrobial susceptibilities, phage types and molecular epidemiology of 89 of these S. enteritidis strains. Fifty per cent were stool isolates whilst 33.3% were isolated from blood samples.
All the isolates were susceptible to cephalothin, ceftriaxone, ciprofloxacin, aztreonam, gentamicin, amikacin, kanamycin, neomycin, streptomycin and nalidixic acid; whilst 75.3% were resistant to sulphonamide, 15.7% to tetracycline, 7.9% to co-trimoxazole, 6.7% to trimethoprim, 2.2% to ampicillin and 2.2% to chloramphenicol. The most frequent phage types were phage type 4 (64%), followed by phage type 1 (12.4%) and phage type 8 (2.2%). Seventy four of the 89 (83.1%) S. enteritidis isolates analysed by pulsed-field gel electrophoresis showed an indistinguishable pattern A when digested by restriction enzyme Xba I suggesting the presence of a predominant clone of S. enteritidis circulating in Singapore in 1997.
肠炎沙门氏菌是新加坡分离出的最常见的非伤寒沙门氏菌，可引起肠胃炎，偶尔还会导致菌血症及继发性并发症。1993年肠炎沙门氏菌分离株数量增加，自那时起，它一直是分离出的最常见沙门氏菌种类。1997年，共分离出139株肠炎沙门氏菌，占所有非伤寒沙门氏菌分离株的19.2%。
我们研究了其中89株肠炎沙门氏菌菌株的抗菌药敏性、噬菌体类型及分子流行病学。50%的菌株分离自粪便，33.3%分离自血液样本。
所有分离株对头孢噻吩、头孢曲松、环丙沙星、氨曲南、庆大霉素、阿米卡星、卡那霉素、新霉素、链霉素和萘啶酸敏感；75.3%对磺胺类耐药，15.7%对四环素耐药，7.9%对复方新诺明耐药，6.7%对甲氧苄啶耐药，2.2%对氨苄西林耐药，2.2%对氯霉素耐药。最常见的噬菌体类型是4型（64%）,其次是1型（12.4%）和8型（2.2%）。通过脉冲场凝胶电泳分析的89株肠炎沙门氏菌分离株中，74株（83.1%）经限制性内切酶Xba I消化后显示出难以区分的A模式，这表明1997年在新加坡流行的肠炎沙门氏菌存在一个主要克隆。
