De Becker G, Moulin V, Pajak B, Bruck C, Francotte M, Thiriart C, Urbain J, Moser M
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rue des Prof. Jeener et Brachet 12, 6041 Gosselies, Belgium.
Int Immunol. 2000 Jun;12(6):807-15. doi: 10.1093/intimm/12.6.807.
The induction of immune responses in vivo is typically performed with antigens administered in external adjuvants, like alum, complete Freund's adjuvant, LPS and, more recently, monophosphoryl lipid A (MPL). However, the role of the adjuvant is still poorly defined. The aim of this study was to test whether the MPL affects the function of antigen-presenting cells (APC) in vitro and in vivo. Antigen-pulsed APC [including macrophages, B cells and dendritic cells (DC)] were incubated or not with MPL, and their ability to sensitize naive T cells was tested in vitro and in vivo. The data show that MPL enhances the ability of macrophages and B cells to sensitize naive T cells, and confers to them the capacity to induce the development of T(h)1 and T(h)2. Administration of MPL i.v. in mice results in the redistribution of fully mature DC in the T cell area of the spleen. These observations suggest that MPL may induce an antigen-specific primary immune response by provoking the migration and maturation of DC that are the physiological adjuvant of the immune system.
体内免疫反应的诱导通常是通过将抗原与外部佐剂一起给药来实现的，这些佐剂包括明矾、完全弗氏佐剂、脂多糖，以及最近的单磷酰脂质A（MPL）。然而，佐剂的作用仍未得到很好的界定。本研究的目的是测试MPL在体外和体内是否会影响抗原呈递细胞（APC）的功能。将抗原脉冲处理的APC[包括巨噬细胞、B细胞和树突状细胞（DC）]与MPL一起孵育或不孵育，并在体外和体内测试它们使未致敏T细胞致敏的能力。数据表明，MPL增强了巨噬细胞和B细胞使未致敏T细胞致敏的能力，并赋予它们诱导T（h）1和T（h）2细胞发育的能力。静脉内给予小鼠MPL会导致完全成熟的DC在脾脏的T细胞区域重新分布。这些观察结果表明，MPL可能通过激发作为免疫系统生理性佐剂的DC的迁移和成熟来诱导抗原特异性的初次免疫反应。
