Khan S H, Baziany A, Banigesh A, Hemmings S J, Shuaib A
Saskatchewan Stroke Research Centre, University of Saskatchewan, Sask., Saskatoon, Canada.
J Neurosci Methods. 2000 May 15;98(1):43-7. doi: 10.1016/s0165-0270(00)00185-0.
This paper presents the determination of an optimal temperature for delayed 2,3,5-triphenyltetrazolium chloride (TTC) staining. Twenty-one rats were subjected to right middle cerebral artery embolic stroke and sacrificed 96 h following ischemia. The brains were harvested and stained immediately after sacrifice or stored for 8 h at 21-23 degrees C or 4 degrees C, respectively. The stained sections were scanned and infarct volume calculated. The quality of staining, distinction of borders between infarcted and non-ischemic tissue and ease of differentiating ischemic tissue in colored and grayscale images was assessed. The present study indicates that results of TTC obtained immediately after animal sacrifice, or delayed TTC staining while storing the brains at room temperature or 4 degrees C are comparable.
本文介绍了确定延迟氯化三苯基四氮唑（TTC）染色的最佳温度。21只大鼠接受右侧大脑中动脉栓塞性卒中，缺血96小时后处死。处死大鼠后立即取出大脑并进行染色，或分别在21-23摄氏度或4摄氏度下保存8小时后进行染色。对染色切片进行扫描并计算梗死体积。评估染色质量、梗死组织与非缺血组织之间边界的清晰度以及在彩色和灰度图像中区分缺血组织的难易程度。本研究表明，动物处死后立即进行TTC检测的结果，或在室温或4摄氏度下保存大脑时进行延迟TTC染色的结果具有可比性。
