Unidad de Investigación, Hospital Universitario de Burgos, Avenida Islas Baleares S/N, Burgos, Spain.
Metab Brain Dis. 2013 Mar;28(1):21-4. doi: 10.1007/s11011-012-9359-x. Epub 2012 Nov 20.
Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it is differentiated from the surrounding normal tissue by staining, usually with 2,3,5-triphenyltetrazolium chloride (TTC), but staining can hamper biochemical colorimetric analysis. We performed this study to avoid this obstacle. A cerebral infarct was provoked in a sample of 10 rats and the brain was cut in coronal sections that were stained with TTC so that the unstained, infarct areas could be delineated in a template of each section in which areas with infarct in all animals were delineated. We calculated infarct coordinates and depth so that the infarct tissue can be sampled without staining. For more precision, the ischemic cortex can be delimited staining its surface before sectioning and cortical tissue into which TTC diffuses can be afterwards discarded, as we had previously measured the TTC diffusion depth in rat brains.
对缺血性脑组织的生化和代谢分析是中风研究的核心，通常在动物中风模型中进行，例如我们使用的大鼠大脑中动脉永久性闭塞 (MCAO)。为了确保样本来自梗死组织，通常使用 2,3,5-三苯基氯化四氮唑 (TTC) 对其进行染色，使其与周围正常组织区分开来，但染色会妨碍生化比色分析。我们进行这项研究是为了避免这个障碍。在 10 只大鼠的样本中引起脑梗死，并对大脑进行冠状切片，用 TTC 染色，以便在每个切片的模板中描绘出未染色的、梗死区域，所有动物的梗死区域都在模板中描绘出来。我们计算了梗死坐标和深度，以便在不染色的情况下取样梗死组织。为了更精确，可以在切片前对缺血皮层进行染色，然后丢弃 TTC 扩散的皮层组织，因为我们之前已经测量了大鼠大脑中 TTC 的扩散深度。
