Heaton M B, Mitchell J J, Paiva M, Walker D W
University of Florida Brain Institute, Department of Neuroscience, Center for Alcohol Research, University of Florida College of Medicine, Box 100244, Gainesville, FL 32610-0244, USA.
Brain Res Dev Brain Res. 2000 May 11;121(1):97-107. doi: 10.1016/s0165-3806(00)00032-8.
Neonatal rats were exposed to ethanol throughout gestation, or during the early postnatal period (postnatal days 4-10 (P4-10)), and enzyme-linked immunoabsorbent assays were subsequently conducted in order to assess nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) protein content in hippocampus, septum, cortex/striatum and cerebellum. These determinations revealed that following prenatal ethanol treatment, there were significant ethanol-induced increases in NGF in P1 cortex/striatum, but no changes in any of the three neurotrophic factors (NTFs) in the other brain regions. Cortex/striatal NGF protein returned to control levels by P10. Following early postnatal exposure, BDNF was elevated in hippocampus and cortex/striatum (assessed on P10), and NGF was also enhanced in cortex/striatum at this age. Hippocampal and cortex/striatal BDNF returned to control levels by P21, but cortex/striatal NGF levels remained enhanced at this age. This NTF did not differ in ethanol and control animals by P60, however. The possible significance of elevated levels of NTFs as a function of ethanol exposure is discussed, and it is speculated that while such alterations could play a protective role, increases in these substances during critical developmental periods could also prove to be deleterious, and could even contribute to certain of the neuropathologies which have been observed following developmental ethanol exposure.
新生大鼠在整个孕期或出生后早期（出生后第4 - 10天（P4 - 10））暴露于乙醇中，随后进行酶联免疫吸附测定，以评估海马体、隔区、皮质/纹状体和小脑中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子-3（NT - 3）的蛋白质含量。这些测定结果显示，产前乙醇处理后，P1期皮质/纹状体中的NGF因乙醇诱导显著增加，但其他脑区的三种神经营养因子（NTF）均无变化。皮质/纹状体中的NGF蛋白在P10时恢复到对照水平。出生后早期暴露后，海马体和皮质/纹状体中的BDNF升高（在P10评估），且该年龄段皮质/纹状体中的NGF也有所增加。海马体和皮质/纹状体中的BDNF在P21时恢复到对照水平，但该年龄段皮质/纹状体中的NGF水平仍保持升高。然而，到P60时，该NTF在乙醇处理组和对照组动物中并无差异。文中讨论了NTF水平升高与乙醇暴露之间关系的潜在意义，并推测虽然这种改变可能起到保护作用，但在关键发育时期这些物质的增加也可能是有害的，甚至可能导致发育性乙醇暴露后观察到的某些神经病理学变化。
