Routledge M N
Department of Biological Sciences, De Montfort University, The Hawthorn Building, The Gateway, LE1 9BH, Leicester, UK.
Mutat Res. 2000 May 30;450(1-2):95-105. doi: 10.1016/s0027-5107(00)00018-x.
Nitric oxide is an important bioregulatory molecule with a range of physiological functions. Nitric oxide can also react with oxygen species to produce a range of reactive nitrogen oxides that can damage DNA and lead to mutations of the DNA base sequence. The mutagenicity of a variety of reactive nitrogen oxide species and related DNA damaging agents in the supF assay are reviewed here, in the context of recent reports that relate to the nature of the DNA lesions responsible for the induced mutations. Mutations induced by nitric oxide in the supF assay are compared to those induced by N(2)O(3), nitrous acid, peroxynitrite and different reactive oxygen species. The effect of replication of the damaged pSP189 plasmid in human cells or Escherichia coli cells is also considered.
一氧化氮是一种具有多种生理功能的重要生物调节分子。一氧化氮还能与氧物种反应生成一系列活性氮氧化物，这些活性氮氧化物会损伤DNA并导致DNA碱基序列突变。本文结合近期有关导致诱导突变的DNA损伤性质的报道，综述了多种活性氮氧化物及相关DNA损伤剂在supF检测中的致突变性。将一氧化氮在supF检测中诱导的突变与N(2)O(3)、亚硝酸、过氧亚硝酸根和不同活性氧物种诱导的突变进行了比较。还考虑了受损的pSP189质粒在人细胞或大肠杆菌细胞中复制的影响。
