Wistuba D, Schurig V
Institut für Organische Chemie, Universität Tübingen, Germany.
J Chromatogr A. 2000 Apr 14;875(1-2):255-76. doi: 10.1016/s0021-9673(00)00066-2.
Enantiomer separation of chiral pharmaceuticals by capillary electrochromatography (CEC) is achieved with open-tubular capillaries (o-CEC), with packed capillaries (p-CEC) or with monolithic capillaries. In o-CEC, capillaries are coated with a thin film containing cyclodextrin derivatives, cellulose, proteins, poly-terguride or molecularly imprinted polymers as chiral selectors. In p-CEC, typical chiral HPLC stationary phases such as silica-bonded cyclodextrin or cellulose derivatives, proteins, glycoproteins, macrocyclic antibiotics, quinine-derived and 'Pirkle' selectors, polyacrylamides and molecularly imprinted polymers are used as chiral selectors. Chiral monolithic stationary phases prepared by in situ polymerization into the capillary were also developed for electrochromatographic enantiomer separation.
通过毛细管电色谱法（CEC）对手性药物进行对映体分离可采用开管毛细管（o-CEC）、填充毛细管（p-CEC）或整体毛细管来实现。在o-CEC中，毛细管涂覆有一层含有环糊精衍生物、纤维素、蛋白质、聚特古立德或分子印迹聚合物作为手性选择剂的薄膜。在p-CEC中，典型的手性高效液相色谱固定相，如硅胶键合环糊精或纤维素衍生物、蛋白质、糖蛋白、大环抗生素、奎宁衍生物和“皮尔克”选择剂、聚丙烯酰胺和分子印迹聚合物被用作手性选择剂。还开发了通过原位聚合进入毛细管制备的手性整体固定相用于电色谱对映体分离。
