Dungelová J, Lehotay J, Rojkovicová T, Cizmárik J
Katedra analytickej chémie Fakulty chemickej a potravinárskej technológie Slovenskej technickej univerzity, Bratislava.
Ceska Slov Farm. 2003 May;52(3):119-25.
Separation of enantiomers by means of high-performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), and capillary electrochromatography (CEC) is of great importance in pharmaceutical analyses. In recent years, separation of various types of racemates employs most frequently chiral stationary phases based on macrocyclic antibiotics. This class of chiral selectors includes vancomycin, teikoplanin, ristocetin A, teikoplanin without saccharide components, avoparcin, etc. The review paper describes the properties of selected antibiotics, the effect of chromatographic conditions on enantioselectivity (effect of the composition of the mobile phase, effect of pH of the mobile phase, effect of temperature), and the study of possible mechanisms of interaction, which play an important role in the separation of enantiomers. Examples of the use of macrocyclic antibiotics in the separation of various compounds by means of the HPLC, SFC, and CEC methods follow.
通过高效液相色谱法（HPLC）、超临界流体色谱法（SFC）和毛细管电色谱法（CEC）分离对映体在药物分析中具有重要意义。近年来，各类外消旋体的分离最常采用基于大环抗生素的手性固定相。这类手性选择剂包括万古霉素、替考拉宁、瑞斯托菌素A、无糖基成分的替考拉宁、阿伏帕星等。这篇综述文章描述了所选抗生素的性质、色谱条件对对映体选择性的影响（流动相组成的影响、流动相pH值的影响、温度的影响）以及可能的相互作用机制的研究，这些在对映体分离中起着重要作用。随后是大环抗生素通过HPLC、SFC和CEC方法用于分离各种化合物的实例。
