Hummel T, Krukkert K, Roos J, Davis G, Klämbt C
Institut für Neurobiologie, Universität Münster, Germany.
Neuron. 2000 May;26(2):357-70. doi: 10.1016/s0896-6273(00)81169-1.
Here we report the description of the Drosophila gene futsch, which encodes a protein recognized by the monoclonal antibody 22C10 that has been widely used to visualize neuronal morphology and axonal projections. The Futsch protein is 5327 amino acids in length. It localizes to the microtubule compartment of the cell and associates with microtubules in vitro. The N- and C-terminal domains of Futsch are homologous to the vertebrate MAP1B microtubule-associated protein. The central domain of the Futsch protein is highly repetitive and shows sequence similarity to neurofilament proteins of which no Drosophila homologs have been reported. Loss-of-function analyses demonstrate that during embryogenesis Futsch is necessary for dendritic and axonal growth. Gain-of-function analyses demonstrate a functional interaction of Futsch with other MAPs. In addition, we show that during development, futsch expression is negatively regulated in nonneuronal tissues.
在此，我们报告果蝇基因futsch的相关描述，该基因编码一种可被单克隆抗体22C10识别的蛋白质，此抗体已被广泛用于观察神经元形态和轴突投射。Futsch蛋白长度为5327个氨基酸。它定位于细胞的微管区室，并在体外与微管结合。Futsch的N端和C端结构域与脊椎动物微管相关蛋白MAP1B同源。Futsch蛋白的中央结构域高度重复，且与神经丝蛋白具有序列相似性，而果蝇中尚未报道有同源物。功能丧失分析表明，在胚胎发育过程中，Futsch对树突和轴突生长是必需的。功能获得分析表明Futsch与其他微管相关蛋白存在功能相互作用。此外，我们还表明，在发育过程中，futsch在非神经组织中的表达受到负调控。
