Henniger M S, Ohl F, Hölter S M, Weissenbacher P, Toschi N, Lörscher P, Wigger A, Spanagel R, Landgraf R
Max Planck Institute of Psychiatry, Kraepelinstr. 2-10, 80804, Munich, Germany.
Behav Brain Res. 2000 Jun 15;111(1-2):153-63. doi: 10.1016/s0166-4328(00)00151-0.
Individuals of high anxiety-related behaviour (HAB) and low anxiety-related behaviour (LAB) rat lines were selectively bred for differences in anxiety-related behaviour on the elevated plus-maze. The goal of this study was to investigate whether this behavioural difference is restricted to the test used as the selection criterion or whether it is a stable and robust trait also in other conflict or non-conflict situations. Therefore, behaviour of male and female HAB and LAB rats was examined in two further tests of unconditioned anxiety: the black-white box and the social interaction test. Furthermore, behaviour of group-housed male HAB and LAB rats was studied in their home cages. In addition to standard statistics, discriminant analyses were performed. The difference in anxiety-related behaviour between the two lines was highly consistent in all tests of unconditioned anxiety. There were also differences in home cage behaviour, LAB rats being more active than HAB rats; this is likely to be a consequence of the LAB rats displaying a higher aggressiveness in social behaviour, compared to HAB rats. In all tests used HAB and LAB rats were clearly distinguished by discriminant analysis. However, while in the elevated plus-maze and the black-white box test the most important parameters for discrimination between the two lines were mainly those generally seen as closely related to anxiety, the discrimination in the social interaction paradigm was primarily due to differences in locomotor activity.
根据高架十字迷宫中焦虑相关行为的差异，选择性培育出高焦虑相关行为（HAB）和低焦虑相关行为（LAB）的大鼠品系。本研究的目的是调查这种行为差异是否仅限于用作选择标准的测试，或者它是否也是其他冲突或非冲突情况下的一种稳定而强大的特征。因此，在另外两种非条件性焦虑测试中检查了雄性和雌性HAB和LAB大鼠的行为：黑白箱测试和社交互动测试。此外，还研究了群居雄性HAB和LAB大鼠在其饲养笼中的行为。除了标准统计分析外，还进行了判别分析。在所有非条件性焦虑测试中，两个品系之间的焦虑相关行为差异高度一致。饲养笼中的行为也存在差异，LAB大鼠比HAB大鼠更活跃；这可能是因为与HAB大鼠相比，LAB大鼠在社交行为中表现出更高的攻击性。在所有使用的测试中，通过判别分析可以清楚地区分HAB和LAB大鼠。然而，在高架十字迷宫和黑白箱测试中，区分两个品系的最重要参数主要是那些通常被认为与焦虑密切相关的参数，而在社交互动范式中的区分主要是由于运动活动的差异。
