Laboratório de Comportamento Exploratório, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Braz J Med Biol Res. 2022 Apr 27;55:e11979. doi: 10.1590/1414-431X2022e11979. eCollection 2022.
Bidirectional selection is a procedure in which an arbitrary characteristic is chosen as a selection criterion and animals exhibiting more of this characteristic are bred in one group and animals exhibiting less are bred in another group. The procedure is repeated along generations until the selected characteristic becomes stable, resulting in two strains that are opposite in relation to the chosen characteristic. The present study aimed at selectively breeding rats exhibiting either a high or a low tendency to socialize by using the proximity test. We tested male and female Wistar rats in a square open field with a communicating birdcage, separated by a grid, containing a co-specific rat and coupled on the outside. Subjects that remained more time in front of the birdcage, interacting with the co-specific rat were bred in a group considered of high sociability (SOC+). Likewise, subjects that remained little time in front of the birdcage, with little interaction with the co-specific rat, were bred in a second group considered of low sociability (SOC-). By the 10th generation, the bidirectional selection resulted in SOC+ rats that spent a large amount of time in front of the cage sniffing and rearing in interaction with the co-specific rat and spent less time in the corners, exploring and grooming. It also resulted in SOC- rats that spent a small amount of time in front of the cage sniffing and rearing in interaction with the co-specific rat and spent more time in the corners and used most of their time grooming.
双向选择是一种程序，其中任意一个特征被选为选择标准，表现出更多这种特征的动物在一个群体中繁殖，表现出较少这种特征的动物在另一个群体中繁殖。这个过程沿着世代重复，直到所选特征变得稳定，从而产生两种在所选特征上相反的品系。本研究旨在通过使用接近测试选择性繁殖表现出高或低社交倾向的大鼠。我们在一个带有连通鸟笼的正方形开阔场地上测试雄性和雌性 Wistar 大鼠，鸟笼被网格隔开，里面有一只同种大鼠，并在外面耦合。那些在鸟笼前停留时间更长、与同种大鼠互动更多的动物被繁殖到一个被认为具有高社交性的群体（SOC+）中。同样，那些在鸟笼前停留时间很短、与同种大鼠互动很少的动物被繁殖到另一个被认为具有低社交性的群体（SOC-）中。到第 10 代，双向选择导致 SOC+大鼠在笼子前花费大量时间嗅探和与同种大鼠互动，并且在角落处花费的时间更少，探索和梳理毛发的时间更少。它还导致 SOC-大鼠在笼子前花费很少的时间嗅探和与同种大鼠互动，并且在角落处花费更多的时间，并且大部分时间都在梳理毛发。
