Palencia C, Hernuss P, Gitsch E, Weghaupt K, Wicke L, Kärcher K H, Steffen C
Wien Klin Wochenschr. 1975 Dec 26;87(24):809-15.
Eleven patients with stage-III cancer of the cervix were investigated before, during and after radio-therapy in regard to their state of humoral immunity on the basis of determinations of the serum IgG, IgM and IgA concentration, of hetero- and isoagglutinins, of tetanus antitoxin before and after vaccination with toxoid, of measles antibodies and of the percentage of lymphocyte membrane fluorescence. The cellular immunity of the same patients was investigated by determination of the percentage of spontaneously-rosetting lymphocytes, of skin-test reactivity with DNCB before and after sensitization, of skin-test reactivity with candida, trichophyton, varidase, OT and staphylo antigen. The function of polymorpho-nuclear leucocytes was investigated by means of the NVT test and St. aureus, E. coli and latex particles. All investigations were performed both before, and 3, 6, 9 and 12 weeks after the commencement of radiotherapy and the results were compared with those of an operated, non-irradiated group (stages I b and II a). Two types of noteworthy results were observed: 1. A decrease in immunological reactivity, probably in connection with cancer, since this reaction was observed both in irradiated and in non-irradiated cases, characterized by lowered or absent immune answer to tetanus toxoid, lymphopenia, decrease in sensitization to DNCB and less positive skin tests to old tuberculin and varidase. 2. An additional inhibition (although in one investigation stimulation of the immune answer was also seen), probably in connection with radiotherapy, characterized by an additional decrease in immune answer to tetanus toxoid, in skin sensitivity to DNCB sensitization and in tests with old tuberculin, and an augmented lymphopenia, as well as an increase in positive skin tests with varidase. No significant changes were observed with any other method.
对11例III期宫颈癌患者在放疗前、放疗期间和放疗后进行了调查，根据血清IgG、IgM和IgA浓度、异种和同种凝集素、类毒素接种前后的破伤风抗毒素、麻疹抗体以及淋巴细胞膜荧光百分比来测定其体液免疫状态。通过测定自发玫瑰花结形成淋巴细胞的百分比、致敏前后对二硝基氯苯（DNCB）的皮肤试验反应性、对念珠菌、毛癣菌、链激酶-链道酶、旧结核菌素（OT）和葡萄球菌抗原的皮肤试验反应性，来研究同一患者的细胞免疫。通过NVT试验以及金黄色葡萄球菌、大肠杆菌和乳胶颗粒来研究多形核白细胞的功能。所有调查均在放疗开始前以及放疗开始后3、6、9和12周进行，并将结果与手术未放疗组（I b期和II a期）的结果进行比较。观察到两种值得注意的结果：1.免疫反应性降低，可能与癌症有关，因为在放疗和未放疗的病例中均观察到这种反应，其特征为对破伤风类毒素的免疫应答降低或缺失、淋巴细胞减少、对DNCB的致敏性降低以及对旧结核菌素和链激酶-链道酶的皮肤试验阳性反应减少。2.额外的抑制作用（尽管在一项调查中也观察到免疫应答的刺激），可能与放疗有关，其特征为对破伤风类毒素的免疫应答进一步降低、对DNCB致敏的皮肤敏感性降低以及对旧结核菌素试验的反应降低，淋巴细胞减少加剧，以及对链激酶-链道酶的皮肤试验阳性反应增加。用任何其他方法均未观察到显著变化。
