Sengupta S, Ray S, Chattopadhyay N, Biswas N, Chatterjee A
Dept. of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, Calcutta, India.
J Exp Clin Cancer Res. 2000 Mar;19(1):81-7.
The intriguing problem of metastasis requires the spreading of metastatic cells through the basement membrane barrier. The interaction of the basement membrane with the metastatic cell is a cell surface activity involving the function of integrin receptors. Integrins are a group of alpha,beta heterodimeric proteins responsible for transducing intracellular signals on binding to the extracellular matrix proteins present in the basement membrane. To understand the role of integrin receptors in tumor metastasis, the cell surface receptor functions were modulated by All Trans Retinoic Acid (ATRA) treatment in B16F10 tumor cells. Our experimental results clearly indicate that All Trans Retinoic Acid (ATRA) inhibit metastatic potential of highly metastatic B16F10 melanoma cells by 1) downregulating the cell surface integrin receptors against ECM proteins specially laminin and vitronectin and 2) by inhibiting the 72 kd collagenase activity.
转移这一引人入胜的问题需要转移性细胞穿过基底膜屏障进行扩散。基底膜与转移性细胞的相互作用是一种细胞表面活性，涉及整合素受体的功能。整合素是一组α、β异二聚体蛋白，负责在与基底膜中存在的细胞外基质蛋白结合时转导细胞内信号。为了了解整合素受体在肿瘤转移中的作用，通过全反式维甲酸（ATRA）处理B16F10肿瘤细胞来调节细胞表面受体功能。我们的实验结果清楚地表明，全反式维甲酸（ATRA）通过以下方式抑制高转移性B16F10黑色素瘤细胞的转移潜能：1）下调针对细胞外基质蛋白特别是层粘连蛋白和玻连蛋白的细胞表面整合素受体；2）抑制72 kd胶原酶活性。
