Stein G
Biomedicine. 1976 May;24(2):106-11.
Blood and bone marrow lymphocytes and cells of lymphoid organs in hematologically normal persons have been studied using free-flow electrophoresis by which a preparative separation of cells according to their electrophoretic mobility is achieved. The results are demonstrated as electrophoretic distributions. Blood lymphocytes have a peak corresponding to relative high electrophoretic mobility, whereas the majority of marrow lymphocytes is found in fractions corresponding to low electrophoretic mobility. The distributions of small lymphocytes from tonsil, spleen and lymphnode either resemble the distribution of blood lymphocytes or have a bimodal fashion with peaks corresponding to blood lymphocytes or marrow lymphocytes. Large lymphoid cells capable of DNA synthesis are concentrated in fractions of intermediate mobility. The results confirm the concept that peripheral T cells have a high electrophoretic mobility whereas most B cells are of low mobility. In contrast to peripheral T cells, cells of the thymus have an intermediate or low electrophoretic mobility. Thus, it appears that in the lymphoid cell system differences in the electrophoretic mobility not only reflect different functional properties but also differences in maturation stages.
利用自由流动电泳对血液学正常个体的血液、骨髓淋巴细胞以及淋巴器官的细胞进行了研究，通过该方法可根据细胞的电泳迁移率对细胞进行制备性分离。结果以电泳分布图展示。血液淋巴细胞有一个对应相对高电泳迁移率的峰，而大多数骨髓淋巴细胞存在于对应低电泳迁移率的组分中。来自扁桃体、脾脏和淋巴结的小淋巴细胞的分布要么类似于血液淋巴细胞的分布，要么呈双峰模式，其峰对应血液淋巴细胞或骨髓淋巴细胞。能够进行DNA合成的大淋巴细胞集中在中等迁移率的组分中。这些结果证实了外周T细胞具有高电泳迁移率而大多数B细胞具有低迁移率这一概念。与外周T细胞相比，胸腺细胞具有中等或低电泳迁移率。因此，在淋巴细胞系统中，电泳迁移率的差异似乎不仅反映了不同的功能特性，还反映了成熟阶段的差异。
