Lavrov V F, Shestakova S V, Simkovskiĭ M V, Golubeva N N
Biull Eksp Biol Med. 1979 Sep;88(9):322-4.
Electrophoretic mobility (EPM) of lymphocytes from the thymus and spleen of August and Wistar rats as well as capacity of lymphocytes with different surface hemagglutinin (PHA) and concanavalin A (Con A) were studied by the method of free flow electrophoresis. Lymphocytes of the rat spleen were shown, depending on the surface charge, to divide into two groups during cultivation: cells with high and low electrophoretic mobility. At separation the lymphocytes consisted of 8--10 fractions with different EPM. There was a relationship between the surface charge of the lymphocytes and their stimulation rate by mitogens. Increased thymidine-3H uptake was recorded at mitogenic exposure of lymphocytes from the spleen with high EPM. Low mobile lymphoid elements of the spleen did not respond to mitogenic stimulation. A subpopulation of thymocytes with low EPM was resistant to Con A stimulation. The thymocytes of rats did not virtually respond to PHA irrespective of EPM.
采用自由流动电泳法研究了八月龄大鼠和Wistar大鼠胸腺及脾脏淋巴细胞的电泳迁移率（EPM），以及不同表面血凝素（PHA）和刀豆球蛋白A（Con A）刺激下淋巴细胞的能力。结果表明，大鼠脾脏淋巴细胞在培养过程中根据表面电荷可分为两组：电泳迁移率高和低的细胞。分离时，淋巴细胞由8 - 10个具有不同EPM的组分组成。淋巴细胞的表面电荷与其对有丝分裂原的刺激率之间存在关联。在高EPM的脾脏淋巴细胞受到有丝分裂原刺激时，记录到3H-胸腺嘧啶核苷摄取增加。脾脏中低迁移率的淋巴细胞对有丝分裂原刺激无反应。低EPM的胸腺细胞亚群对Con A刺激具有抗性。大鼠胸腺细胞无论EPM如何，实际上对PHA均无反应。
