Yang S L, Zaneveld L J, Schumacher G F
Fertil Steril. 1976 May;27(5):577-81.
The in vivo effect of purified human alpha1-antitrypsin and inter-alpha-trypsin inhibitor on the fertilizing ability of capacitated rabbit spermatozoa was investigated and compared with that of the trypsin-kallikrein inhibitor from bovine organs (Trasylol). Only 250 mug of alpha1-antitrypsin/5 X 10(4) sperm in 0.05 ml inhibited fertilization by more than 50%. Lower concentrations of alpha1-antitrypsin (50 mug/5 X 10(4) sperm in 0.05 mo) and inter-alpha-trypsin inhibitor in amounts of 250 mug/5 X 10(4) sperm and less had no significant effect on the fertilization rate. Comparative experiments with Trasylol in conentrations of 50 mug/5 X 10(4) sperm resulted in more than 50% fertilization inhibition, whereas 100 mug/5 X 10(4) sperm decreased the fertilization rate by approximately 90%. The differences in the antifertility effect of these acrosin inhibitors toward capacitated spermatozoa may be due to differences in molecular weight. The effective concentrations required for fertilization inhibition appear to be relatively high under the experimental conditions used.
研究了纯化的人α1 -抗胰蛋白酶和α-胰蛋白酶抑制剂对获能兔精子受精能力的体内作用，并与牛器官中的胰蛋白酶 -激肽释放酶抑制剂（抑肽酶）进行了比较。在0.05 ml中，每5×10⁴个精子仅250μg的α1 -抗胰蛋白酶就能抑制超过50%的受精。较低浓度的α1 -抗胰蛋白酶（0.05 ml中每5×10⁴个精子50μg）和α-胰蛋白酶抑制剂，用量为每5×10⁴个精子250μg及更低剂量时，对受精率无显著影响。用浓度为每5×10⁴个精子50μg的抑肽酶进行的对比实验导致超过50%的受精抑制，而每5×10⁴个精子100μg则使受精率降低约90%。这些顶体蛋白酶抑制剂对获能精子的抗生育作用差异可能归因于分子量的不同。在所使用的实验条件下，抑制受精所需的有效浓度似乎相对较高。
