Parrish J J, Susko-Parrish J L, Handrow R R, Ax R L, First N L
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
Gamete Res. 1989 Dec;24(4):403-13. doi: 10.1002/mrd.1120240407.
The effects of sulfated glycoconjugates on the preparation of mammalian sperm for fertilization were investigated. The three sulfated glycoconjugates tested were heparin, dextran sulfate, and the fucose sulfate glycoconjugate (FSG) from the sea urchin egg jelly coat. In vivo, FSG induces the acrosome reaction in sea urchin sperm. Bovine sperm were found to be capacitated by heparin and FSG as judged both by ability of lysophosphatidylcholine (LC) to induce an acrosome reaction and by ability to fertilize bovine oocytes in vitro. The mechanism by which heparin or FSG capacitated bovine sperm appeared similar, since glucose inhibited capacitation by both glycoconjugates. In contrast to effects on bovine sperm, heparin and FSG induced the acrosome reaction in capacitated hamster sperm. When hamster sperm were incubated under noncapacitating conditions, heparin had no effect on capacitation or the acrosome reaction. Three molecular weights (MW) of dextran sulfate (5,000, 8,000, 500,000) were found to capacitate bovine sperm as judged by the ability of LC to induce an acrosome reaction. Whereas bovine sperm incubated with 5,000 or 8,000 MW dextran sulfate fertilized more bovine oocytes than control sperm (P less than 0.05), sperm treated with 500,000 MW dextran sulfate failed to penetrate oocytes. The high-MW dextran sulfate appeared to interact with the zona pellucida and/or sperm to prevent sperm binding. Results suggest that sulfated glycoconjugates may prepare sperm for fertilization across a wide range of species.
研究了硫酸化糖缀合物对哺乳动物精子受精准备的影响。所测试的三种硫酸化糖缀合物分别是肝素、硫酸葡聚糖以及来自海胆卵黄膜的硫酸岩藻糖糖缀合物（FSG）。在体内，FSG可诱导海胆精子发生顶体反应。通过溶血磷脂酰胆碱（LC）诱导顶体反应的能力以及体外使牛卵母细胞受精的能力判断，发现肝素和FSG可使牛精子获能。肝素或FSG使牛精子获能的机制似乎相似，因为葡萄糖可抑制这两种糖缀合物的获能作用。与对牛精子的作用不同，肝素和FSG可诱导已获能的仓鼠精子发生顶体反应。当仓鼠精子在非获能条件下孵育时，肝素对获能或顶体反应无影响。通过LC诱导顶体反应的能力判断，发现三种分子量（MW）的硫酸葡聚糖（5000、8000、500000）均可使牛精子获能。与对照精子相比，用5000或8000 MW硫酸葡聚糖孵育的牛精子使更多的牛卵母细胞受精（P小于0.05），而用500000 MW硫酸葡聚糖处理的精子未能穿透卵母细胞。高分子量的硫酸葡聚糖似乎与透明带和/或精子相互作用以阻止精子结合。结果表明，硫酸化糖缀合物可能使多种物种的精子为受精做好准备。
