Manachini P L, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina M G
Int J Syst Evol Microbiol. 2000 May;50 Pt 3:1331-1337. doi: 10.1099/00207713-50-3-1331.
A polyphasic study was performed on 10 soil isolates of thermophilic denitrifying Bacillus strains from different geographical areas. The presence of two main characteristic bands following amplification of the internal transcribed spacer (ITS) region of rrn operons suggests a close relatedness to 'Bacillus thermodenitrificans'. The isolates cluster around two strains of 'B. thermodenitrificans' in riboprint and fatty acid analyses, though differences occur at the strain level. Subsequent DNA-DNA reassociation studies including the 10 isolates, 'B. thermodenitrificans' DSM 465T and DSM 466, and Bacillus stearothermophilus ATCC 12980T and Bacillus thermoleovorans ATCC 43513T revealed such a high level of genomic relatedness between the isolates and the DSM strains (> 73% similarity) that they must be considered strains of the same taxon. The degree of DNA-DNA similarity between the 12 strains of 'B. thermodenitrificans' and the type strains of the other two phylogenetically neighbouring Bacillus species was significantly lower (21-43% similarity). Based upon phylogenetic, chemotaxonomic and phenotypic evidence, the designation of B. thermodenitrificans sp. nov., nom. rev. is proposed. The type strain of B. thermodenitrificans is DSM 465T.
对来自不同地理区域的10株嗜热反硝化芽孢杆菌土壤分离株进行了多相研究。rrn操纵子的内转录间隔区（ITS）区域扩增后出现两条主要特征带，表明这些分离株与“热脱硝芽孢杆菌”密切相关。在核糖体印记和脂肪酸分析中，这些分离株聚集在两株“热脱硝芽孢杆菌”周围，不过在菌株水平上存在差异。随后的DNA-DNA重缔合研究包括这10株分离株、“热脱硝芽孢杆菌”DSM 465T和DSM 466，以及嗜热脂肪芽孢杆菌ATCC 12980T和嗜热解油芽孢杆菌ATCC 43513T，结果显示这些分离株与DSM菌株之间的基因组相关性水平很高（相似度>73%），因此必须将它们视为同一分类单元的菌株。12株“热脱硝芽孢杆菌”与其他两个系统发育相邻芽孢杆菌物种的模式菌株之间的DNA-DNA相似程度明显较低（相似度为21%-43%）。基于系统发育、化学分类和表型证据，提出了热脱硝芽孢杆菌新种（B. thermodenitrificans sp. nov.），名称修正（nom. rev.）。热脱硝芽孢杆菌的模式菌株为DSM 465T。
