Graves C N, Sanders S S, Shoemaker R L, Rehm W S
J Appl Physiol. 1976 Mar;40(3):447-50. doi: 10.1152/jappl.1976.40.3.447.
An intact globe method was developed to determine the characteristics of the cornea of the bullfrog, Rana catesbeiana. With this method the anterior chamber could be perfused and the transcorneal potential difference (PD) and electrical resistance determined. It was found for the endothelium plus stroma (epithelium scraped) that the PD was essentially zero and the electrical resistance was only a small fraction of that of the intact cornea. Elevation of K+ or decrease in Cl- concentration in the anterior chamber produced in intact corneas a large and rapid change in PD while with the epithelium scraped (stroma and endothelium intact) these elevations produced a negligible change in PD. It is concluded that ions can rapidly move across the endothelium and stroma of the cornea.
一种完整眼球法被开发出来以确定牛蛙（牛蛙，虎纹蛙）角膜的特性。使用这种方法，可以对前房进行灌注，并测定跨角膜电位差（PD）和电阻。研究发现，对于内皮加基质（上皮刮除），PD基本为零，电阻仅为完整角膜的一小部分。在前房中升高K+或降低Cl-浓度，在完整角膜中会使PD产生大而迅速的变化，而在上皮刮除（基质和内皮完整）时，这些升高对PD产生的变化可忽略不计。结论是离子可以快速穿过角膜的内皮和基质。
