通过聚丙烯酰胺凝胶等电聚焦法测定α1-抗胰蛋白酶表型
Determination of alpha1-antitrypsin phenotypes by isoelectric focusing in polyacrylamide gels.
作者信息
Kueppers F
出版信息
J Lab Clin Med. 1976 Jul;88(1):151-5.
PMID:1084381
Abstract
A method for alpha1-antitrypsin phenotyping is described that uses isoelectric focusing in a pH gradient 3.5 to 5 with polyacrylamide as supporting medium. The method is fast, reproducible, and reliable and offers some advantages over the currently used starch-gel electrophoresis.
摘要
本文描述了一种α1-抗胰蛋白酶表型分析方法，该方法在以聚丙烯酰胺为支持介质、pH值为3.5至5的梯度中进行等电聚焦。该方法快速、可重复且可靠，与目前使用的淀粉凝胶电泳相比具有一些优势。
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