通过毛细管等电聚焦法测定血清中的α1-抗胰蛋白酶。
Alpha1-antitrypsin in serum determined by capillary isoelectric focusing.
作者信息
Lupi A, Viglio S, Luisetti M, Gorrini M, Coni P, Faa G, Cetta G, Iadarola P
机构信息
Dipartimento di Biochemica A. Castellani, Università di Pavia, Italy.
出版信息
Electrophoresis. 2000 Sep;21(15):3318-26. doi: 10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I.
A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (alpha1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose alpha1AT phenotype was previously identified and of twelve subjects whose alpha1AT phenotype was unknown. The results obtained allowed us to confirm or identify the alpha1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of alpha1AT variants associated with diseases of clinical relevance.
已开发出一种毛细管等电聚焦（CIEF）方法，该方法使用填充有聚环氧乙烷（PEO）和pH值在3.5 - 5.0范围内的载体两性电解质溶液的裸熔融石英毛细管，用于鉴定人血清中的α1 - 抗胰蛋白酶（α1AT）表型。这种新方法常规应用于对五名先前已鉴定α1AT表型的对照者以及十二名α1AT表型未知的受试者的血清样本进行研究。所获得的结果使我们能够在所有测试血清中确认或鉴定α1AT表型。该方法似乎特别适合于鉴定与具有临床相关性的疾病相关的α1AT变体。
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