在患有睾丸功能衰竭的不育成年男性和青春期前男孩血清中存在的抗精子抗体所识别的精子抗原。

Sperm antigens recognized by antisperm antibodies present in sera of infertile adults and prepubertal boys with testicular failure.

作者信息

Domagała A, Kamieniczna M, Kurpisz M

机构信息

Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland.

出版信息

Int J Androl. 2000 Jun;23(3):150-5. doi: 10.1046/j.1365-2605.2000.00219.x.

DOI:10.1046/j.1365-2605.2000.00219.x

PMID:10844540

Abstract

Immunoblotting of a repertoire of sperm antigens reacting with antisperm antibodies present in sera of infertile adults and prepubertal boys with testicular failure was performed. In the subgroups selected for this study, 55% of examined infertile women, 65% of infertile men and 64% of prepubertal boys with gonadal failure gave positive results by Western blotting with extracted sperm antigens. Sperm antigens with molecular weights of 57, 58, 62, 63 and 66 kDa were the most immunodominant entities recognized by antisperm antibodies from prepubertal boys. No positive reactions were detected by Western blotting in a control population of fertile adults, whereas in a group of prepubertal healthy boys only one sample revealed reactivity against sperm antigens of 58 and 70 kDa.

摘要

对不育成年男性和患有睾丸功能衰竭的青春期前男孩血清中存在的抗精子抗体所反应的一系列精子抗原进行了免疫印迹分析。在本研究选定的亚组中，55%的受试不育女性、65%的不育男性和64%患有性腺功能衰竭的青春期前男孩通过对提取的精子抗原进行蛋白质印迹法检测得到阳性结果。分子量为57、58、62、63和66 kDa的精子抗原是青春期前男孩的抗精子抗体识别的最主要免疫优势实体。在可育成年男性对照人群中，蛋白质印迹法未检测到阳性反应，而在一组青春期前健康男孩中，只有一个样本显示出对58和70 kDa精子抗原的反应性。