Bienzle D, McDonnell J J, Stanton J B
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602-7388, USA.
J Am Vet Med Assoc. 2000 Jun 1;216(11):1761-4. doi: 10.2460/javma.2000.216.1761.
To compare differential cell counts and cell characteristics of CSF samples analyzed immediately or after storage for 24 and 48 hours at 4 C with and without the addition of autologous serum.
Prospective study.
36 dogs and 6 cats.
CSF samples were collected from the cerebellomedullary cistern and divided into 250-microliter aliquots. Slides of CSF samples were prepared by use of cytocentrifugation immediately and after 24 and 48 hours of storage with addition of autologous serum (final concentrations, 11 and 29%). Differential cell counts and number of unrecognizable cells were compared among preparations.
Significant differences in the differential cell counts were not detected among samples analyzed before or after storage. Although the number of unrecognizable cells increased with storage time, this did not result in a significant effect on cell distribution or diagnosis. Cells in CSF samples stored with 11% serum more closely resembled cells in fresh samples than did cells in samples stored with 29% serum.
CSF samples collected at veterinary clinics remote from a diagnostic laboratory or during nonoperational hours may be preserved through the addition of autologous serum. Evaluation of such samples is likely to result in an accurate diagnosis for at least 48 hours after collection.
比较在添加或不添加自体血清的情况下，立即分析的脑脊液（CSF）样本以及在4℃下储存24小时和48小时后的CSF样本的细胞分类计数和细胞特征。
前瞻性研究。
36只狗和6只猫。
从小脑延髓池采集CSF样本，并分成250微升的等分试样。通过细胞离心法立即制备CSF样本的玻片，并在添加自体血清（终浓度分别为11%和29%）后储存24小时和48小时后制备玻片。比较各制剂之间的细胞分类计数和无法识别的细胞数量。
在储存前后分析的样本之间未检测到细胞分类计数的显著差异。虽然无法识别的细胞数量随储存时间增加，但这对细胞分布或诊断没有显著影响。与储存于29%血清中的样本相比，储存于11%血清中的CSF样本中的细胞更类似于新鲜样本中的细胞。
在远离诊断实验室的兽医诊所或非工作时间采集的CSF样本可通过添加自体血清来保存。对这些样本的评估可能会在采集后至少48小时得出准确诊断。
