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[犬猫脑脊液细胞富集沉淀技术的经验。第1部分]

[Experiences with a sedimentation technique for the enrichment of cerebrospinal fluid cells in the dog and cat. Part 1].

作者信息

Grevel V

机构信息

Klinik und Poliklinik für kleine Haustiere an der Freien Universität Berlin.

出版信息

Tierarztl Prax. 1991 Oct;19(5):553-60.

PMID:1759272
Abstract

The differentiation of cells of the cerebrospinal fluid (CSF) gives valuable information about certain neurologic diseases. The sedimentation technique of Sayk modified by Kölmel is introduced and its application described. Cells of 300 samples of CSF from dogs and cats are evaluated. There were very good to reasonable results in 88% (263 of 300) of the samples. A comparison between cell number, morphology and protein content in 150 samples was performed. 90 CSF samples with normal cell count (less than or equal to 5/mm3, determined in the Fuchs-Rosenthal chamber) were compared with the cell yield after sedimentation. In 58% (52 of 90) of the samples more than 200 cells were found, in 13% (12 of 90) more than 800 could be differentiated. The results are compared with those of other methods mentioned in the literature.

摘要

脑脊液(CSF)细胞的分化可为某些神经系统疾病提供有价值的信息。介绍了经科尔梅尔改良的赛克沉降技术及其应用。对来自狗和猫的300份脑脊液样本的细胞进行了评估。88%(300份样本中的263份)的样本取得了非常好到合理的结果。对150份样本的细胞数量、形态和蛋白质含量进行了比较。将90份细胞计数正常(在富克斯-罗森塔尔计数板中测定,小于或等于5/mm³)的脑脊液样本与沉降后的细胞产量进行了比较。在58%(90份样本中的52份)的样本中发现了200多个细胞,在13%(90份样本中的12份)的样本中可分辨出800多个细胞。将结果与文献中提到的其他方法的结果进行了比较。

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