Chiang M T, Chang S Y, Whang C W
Department of Chemistry, Tunghai University, Taichung, Taiwan.
J Chromatogr A. 2000 Apr 28;877(1-2):233-7. doi: 10.1016/s0021-9673(00)00168-0.
A new analytical method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and a He-Cd laser (excitation at 442 nm, emission at 500 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the concentration limit of detection was in the nanomolar level. Coupled with a simple cleanup procedure, the method was successfully applied to the analysis of baclofen in human plasma. Recovery of spiked baclofen in plasma was 98%. The relative standard deviation values on peak size and migration time were 7.9% and 0.4%, respectively. The limit of detection of baclofen in plasma was 10 ng/ml.
已开发出一种基于毛细管电泳分离和激光诱导荧光检测的巴氯芬（4-氨基-3-对氯苯基丁酸）分析新方法。萘-2,3-二甲醛用于对这种无荧光药物进行柱前衍生化。使用50 mM硼酸钠（pH 9.5）的电泳缓冲液和氦镉激光器（激发波长442 nm，发射波长500 nm）可实现最佳分离和检测。通常在三个数量级上获得线性关系（r≥0.99），检测限浓度处于纳摩尔水平。结合简单的净化程序，该方法成功应用于人血浆中巴氯芬的分析。血浆中添加的巴氯芬回收率为98%。峰面积和迁移时间的相对标准偏差值分别为7.9%和0.4%。血浆中巴氯芬的检测限为10 ng/ml。
