Gu Yi-Sheng, Whang Chen-Wen
Department of Chemistry, Tunghai University, Taichung, Taiwan.
J Chromatogr A. 2002 Oct 4;972(2):289-93. doi: 10.1016/s0021-9673(02)01083-x.
A capillary electrophoretic method with laser-induced fluorescence detection for baclofen (4-amino-3-p-chlorophenylbutyric acid) has been developed. 6-Carboxyfluorescein succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the lowest derivatizable concentration limit for baclofen in aqueous solution was 10 nM (2 ng baclofen/ml). Coupled with a simple clean up procedure, the method can be applied to the analysis of baclofen in human plasma at micromolar level. Recovery of spiked baclofen in plasma was 95%. The relative standard deviation values on peak size (0.5 microM level) and migration time were 8.2 and 1.0% (n=7), respectively. The limit of detection of baclofen in plasma was 0.1 microM (21 ng/ml).
已开发出一种采用激光诱导荧光检测的毛细管电泳法来测定巴氯芬（4-氨基-3-对氯苯基丁酸）。6-羧基荧光素琥珀酰亚胺酯用于对这种无荧光的药物进行柱前衍生化。使用50 mM硼酸钠（pH 9.5）的电泳缓冲液和空气冷却的氩离子激光器（激发波长488 nm，发射波长520 nm）可实现最佳的分离和检测。通常在三个数量级范围内可获得线性关系（r≥0.99），水溶液中巴氯芬的最低可衍生化浓度极限为10 nM（2 ng巴氯芬/毫升）。结合简单的净化程序，该方法可用于分析微摩尔水平的人血浆中的巴氯芬。血浆中加标的巴氯芬回收率为95%。峰面积（0.5 microM水平）和迁移时间的相对标准偏差值分别为8.2%和1.0%（n = 7）。血浆中巴氯芬检测限为0.1 microM（21 ng/毫升）。
