Zhong X Y, Holzgreve W, Hahn S
Department of Obstetrics and Gynaecology, University of Basel, Switzerland.
BJOG. 2000 Jun;107(6):766-9. doi: 10.1111/j.1471-0528.2000.tb13338.x.
To test the sensitivity, specificity and reproducibility using fetal DNA obtained from plasma of pregnant women by polymerase chain reaction for the simultaneous detection of both fetal sex and Rhesus D genotype.
Blood samples were obtained from 22 Rhesus D negative pregnant women about to undergo an invasive procedure. DNA was extracted from the plasma fraction and analysed by a multiplex nested polymerase chain reaction using Y chromosome-and Rhesus D-specific primers. The results of this experimental procedure were compared with those obtained from the analysis performed on material gained by the invasive procedure.
The sensitivity of the plasma polymerase chain reaction-based method was surprisingly high, with both fetal genotypes being correctly determined in almost 100% of the cases examined. In only one instance was a false positive result for the detection of Rhesus D recorded, which on subsequent analysis was negative.
The ease and rapidity with which the plasma polymerase chain reaction-based method can be performed makes it a promising method for the analysis of multiple fetal loci, such as Rhesus D and sex.
通过聚合酶链反应，利用从孕妇血浆中获取的胎儿DNA同时检测胎儿性别和恒河猴D基因型，以测试该方法的敏感性、特异性和可重复性。
从22名即将接受侵入性操作的恒河猴D阴性孕妇采集血样。从血浆部分提取DNA，并使用Y染色体和恒河猴D特异性引物通过多重巢式聚合酶链反应进行分析。将该实验程序的结果与通过侵入性操作获得的材料分析结果进行比较。
基于血浆聚合酶链反应的方法敏感性出奇地高，在所检测的几乎100%的病例中，两种胎儿基因型均被正确判定。仅在一例中记录到恒河猴D检测的假阳性结果，后续分析显示为阴性。
基于血浆聚合酶链反应的方法操作简便快捷，使其成为分析多个胎儿基因座（如恒河猴D和性别）的有前景的方法。
