Benlali A, Draskovic I, Hazelett D J, Treisman J E
Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York 10016, USA.
Cell. 2000 Apr 28;101(3):271-81. doi: 10.1016/s0092-8674(00)80837-5.
Cells in the morphogenetic furrow of the Drosophila eye disc undergo a striking shape change immediately prior to their neuronal differentiation. We have isolated mutations in a novel gene, act up (acu), that is required for this shape change. acu encodes a homolog of yeast cyclase-associated protein, which sequesters monomeric actin; we show that acu is required to prevent actin filament polymerization in the eye disc. In contrast, profilin promotes actin filament polymerization, acting epistatically to acu. However, both acu and profilin are required to prevent premature Hedgehog-induced photoreceptor differentiation ahead of the morphogenetic furrow. These findings suggest that dynamic changes in actin filaments alter cell shape to control the movement of signals that coordinate a wave of differentiation.
果蝇眼盘形态发生沟中的细胞在神经元分化之前会经历显著的形态变化。我们分离出了一个新基因act up（acu）中的突变，这种形态变化需要该基因。acu编码酵母环化酶相关蛋白的同源物，该蛋白隔离单体肌动蛋白；我们发现acu是防止眼盘中肌动蛋白丝聚合所必需的。相比之下，原肌球蛋白促进肌动蛋白丝聚合，其作用在acu之上。然而，acu和原肌球蛋白都是防止形态发生沟前方过早出现由刺猬蛋白诱导的光感受器分化所必需的。这些发现表明，肌动蛋白丝的动态变化改变细胞形态，以控制协调分化波的信号的移动。
