Boquet I, Boujemaa R, Carlier M F, Préat T
Laboratoire Développement, Evolution, et Plasticité du Système Nerveux, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette, France.
Cell. 2000 Sep 15;102(6):797-808. doi: 10.1016/s0092-8674(00)00068-4.
A dynamic actin cytoskeleton is essential for the remodeling of cell shape during development, but the specific roles of many actin partners remain unclear. Here we characterize a novel actin binding protein, Ciboulot (Cib), which plays a major role in axonal growth during Drosophila brain metamorphosis. Loss of Cib function leads to axonal growth defects in the central brain, while overexpression of the gene during development leads to overgrown projections. The Cib protein displays strong sequence similarity to beta-thymosins but has biochemical properties like profilin: the Cib-actin complex participates in actin filament assembly exclusively at the barbed end, and Cib enhances actin-based motility in vitro. Genetic experiments show that Cib and the Drosophila profilin protein Chickadee (Chic) cooperate in central brain metamorphosis.
动态肌动蛋白细胞骨架对于发育过程中细胞形状的重塑至关重要，但许多肌动蛋白结合伙伴的具体作用仍不清楚。在这里，我们描述了一种新型肌动蛋白结合蛋白，Ciboulot（Cib），它在果蝇大脑变态过程中的轴突生长中起主要作用。Cib功能丧失会导致中枢脑轴突生长缺陷，而在发育过程中该基因的过表达会导致投射过度生长。Cib蛋白与β-胸腺素显示出很强的序列相似性，但具有类似于肌动蛋白解聚因子的生化特性：Cib-肌动蛋白复合物仅在肌动蛋白丝的尖端参与肌动蛋白丝组装，并且Cib在体外增强基于肌动蛋白的运动。遗传学实验表明，Cib与果蝇肌动蛋白解聚因子蛋白Chickadee（Chic）在中枢脑变态过程中协同作用。
