Asherson G L, Mayhew B
Isr J Med Sci. 1976 Apr-May;12(4-5):454-67.
This paper describes an investigation of why contact sensitizing agents cause strong cell-mediated immunity. Contact sensitivity was induced in mice by painting the skin with 4-ethoxymethylene-2-phenyloxazolone (oxazolone), and measured by the increase of ear thickness following challenge six days later. Reactivity was transferred by taking the regional lymph node cells from mice 18 h after immunization and injecting them into the footpads of recipients. This "18-h transfer" has several characteristics. As few as 2 X 10(4) cells were effective. The donor lymph node cells were best taken one to three days after immunization, were less effective on day 4 and virtually inactive by day 7. The recipients developed contact sensitivity when challenged on day 4, but lacked sensitivity when challenged on days 1 and 2 after transfer. The transferred cells were still active after treatment with anti-theta serum and complement. They also resisted 2,000 R in vitro, mitomycin, vinblastine, and inhibitors of protein synthesis such as emetine, cycloheximide and puromycin. The transfer was prevented by treatment with trypsin, freeze-thawing, and heating at 56 C. Plasma membranes were also immunogenic. The evidence suggests that the "18-h transfer" is a special type of active immunization, not due to ordinary free oxazolone, and that the agent is present within the lymph node in a free oxazolone, and that the agent is present within the lymph node in a specially immunogenic location or form.
本文描述了一项关于接触致敏剂为何会引发强烈细胞介导免疫反应的研究。通过用4-乙氧基亚甲基-2-苯基恶唑酮（恶唑酮）涂抹小鼠皮肤诱导接触敏感性，并在六天后再次接触后通过耳部厚度增加来测量。在免疫18小时后从小鼠获取区域淋巴结细胞并将其注射到受体的脚垫中，以此来转移反应性。这种“18小时转移”具有几个特点。低至2×10⁴个细胞就有效。供体淋巴结细胞最好在免疫后1至3天获取，在第4天效果较差，到第7天几乎无活性。受体在转移后第4天再次接触时会产生接触敏感性，但在转移后第1天和第2天再次接触时则缺乏敏感性。转移的细胞在用抗θ血清和补体处理后仍有活性。它们在体外还能抵抗2000伦琴辐射、丝裂霉素、长春碱以及蛋白质合成抑制剂如依米丁、环己酰亚胺和嘌呤霉素。用胰蛋白酶处理、冻融以及在56℃加热可阻止这种转移。质膜也具有免疫原性。证据表明“18小时转移”是一种特殊类型的主动免疫，并非由于普通的游离恶唑酮，而且该物质以游离恶唑酮的形式存在于淋巴结内，并且在淋巴结内处于一种特殊的免疫原性位置或形式。
