Macatonia S E, Edwards A J, Knight S C
Immunology. 1986 Dec;59(4):509-14.
Lymph node cells taken 24 hr after skin-painting mice with the contact sensitizer fluorescein isothiocyanate (FITC) induce delayed-type hypersensitivity in recipient mice. Skin-painting increased the number of dendritic cells (DC) in the draining lymph nodes without significantly changing the number of lymphocytes at 24 hr. The antigen was preferentially located on the DC. Raising the dose of FITC increased both the number of DC and the amount per cell. The addition of these DC to syngeneic lymph node cells at a ratio as low as 1:300 initiated proliferative responses in vitro. The level of proliferation was related to the amount of antigen on the DC. Mice given 50,000 of these fluorescent DC developed specific contact sensitivity reactions. DC exposed in vitro to FITC also acquired antigen and were able to initiate proliferative responses in vitro and to sensitize recipient mice. The DC may therefore be the prime cell involved in the induction of delayed-type hypersensitivity.
在用接触性致敏剂异硫氰酸荧光素（FITC）给小鼠皮肤涂抹24小时后获取的淋巴结细胞，可在受体小鼠中诱导迟发型超敏反应。皮肤涂抹在24小时时增加了引流淋巴结中树突状细胞（DC）的数量，而淋巴细胞数量没有显著变化。抗原优先定位在DC上。提高FITC的剂量会增加DC的数量以及每个细胞上的抗原量。以低至1:300的比例将这些DC添加到同基因淋巴结细胞中，可在体外引发增殖反应。增殖水平与DC上的抗原量有关。给予50,000个这些荧光DC的小鼠产生了特异性接触敏感性反应。体外暴露于FITC的DC也获取了抗原，并且能够在体外引发增殖反应并使受体小鼠致敏。因此，DC可能是参与迟发型超敏反应诱导的主要细胞。
