Nasledov G A, Radziukevich T L
Tsitologiia. 1975 Dec;17(12):1383-8.
Effect of glycerol, sucrose and NaC1 hypertonic solutions exerted on potassium contracture and resting membrane potential in isolated frog tonic muscle fibres were studied. Sucrose and NaC1 hypertonic solutions induce a stable depolarization and depress potassium contracture, while glycerol results in a transient depolarization followed by recovery of resting membrane potential and contracture reactions. The removal of 400 mM glycerol induces an insignificant depolarization and disappearance of contracture reactions, however, after the removal of sucrose of NaC1 hypertonicity both the parameters tend to restore. The isolated phasic and tonic fibres lose irreversibly potassium contracture reactions after the removal of 100 mM and 400 mM glycerol, respectively, the latter being more resistant to this procedure. Nevertheless it is concluded that the T-system is equally important for excitation--contraction coupling in tonic and in phasic fibres.
研究了甘油、蔗糖和氯化钠高渗溶液对离体青蛙强直性肌纤维钾挛缩和静息膜电位的影响。蔗糖和氯化钠高渗溶液可诱导稳定的去极化并抑制钾挛缩，而甘油则导致短暂的去极化，随后静息膜电位和挛缩反应恢复。去除400 mM甘油会引起不显著的去极化和挛缩反应消失，然而，去除蔗糖或氯化钠高渗性后，这两个参数都趋于恢复。分别去除100 mM和400 mM甘油后，离体的相位性和强直性纤维不可逆地失去钾挛缩反应，后者对该过程更具抵抗力。然而，可以得出结论，T系统对于强直性和相位性纤维的兴奋 - 收缩偶联同样重要。
