Brennan P A, Palacios-Callender M, Sinclair D, Spedding A V, Zaki G A
Queen Alexandra Hospital, Portsmouth, UK.
J Craniomaxillofac Surg. 2000 Feb;28(1):44-8. doi: 10.1054/jcms.2000.0108.
The small molecule nitric oxide (NO) has generated an exponential amount of research since its discovery as a biological messenger in 1987. It has a vast number of actions, many of which are poorly understood. It has been studied in a variety of human cancers and has been implicated both in tumour promotion and inhibition. Although NO is produced by three distinct isoforms of the enzyme nitric oxide synthase (NOS), most cancer research is directed towards the calcium-independent form, iNOS which following induction, produces much higher quantities of NO than the other two. In this study the expression of iNOS is assessed by immunohistochemistry in 26 cases of oral epithelial dysplasia ranging in severity from mild to severe. iNOS staining was found in all 26 cases of dysplasia with the degree of staining correlating to the severity of dysplasia (p < 0.001). There was no iNOS staining seen in adjacent normal epithelium. The possible role of iNOS in the complex transformation from dysplasia to invasive oral cancer and the clinical applications are discussed.
自1987年小分子一氧化氮（NO）作为一种生物信使被发现以来，对其的研究呈指数级增长。它具有大量的作用，其中许多作用还了解甚少。它已在多种人类癌症中得到研究，并且在肿瘤促进和抑制方面都有涉及。尽管NO由一氧化氮合酶（NOS）的三种不同同工型产生，但大多数癌症研究都针对不依赖钙的形式，即诱导型一氧化氮合酶（iNOS），诱导后，它产生的NO量比其他两种同工型高得多。在本研究中，通过免疫组织化学评估了26例从轻度到重度的口腔上皮发育异常病例中iNOS的表达。在所有26例发育异常病例中均发现iNOS染色，染色程度与发育异常的严重程度相关（p < 0.001）。在相邻的正常上皮中未见到iNOS染色。讨论了iNOS在从发育异常到侵袭性口腔癌的复杂转变中的可能作用以及临床应用。
