Brennan P A, Conroy B, Spedding A V
Maxillofacial Department, Queen Alexandra Hospital, Portsmouth, UK.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2000 Nov;90(5):624-9. doi: 10.1067/moe.2000.108800.
Nitric oxide (NO) has been studied in a variety of human cancers and is implicated in both tumor promotion and inhibition. Downregulation of the enzyme iNOS by wild-type p53 (but not mutant) protein has been shown to occur in normal cells and some tumors, but the relationship has not been reported in oral epithelial dysplasia.
An immunohistochemical study was conducted with antibodies to iNOS and p53 (clone DO-7) in 36 cases of oral dysplasia of varying severity. Statistical analysis showed a significant correlation between iNOS staining and grade of dysplasia (P <.001) and between p53 and iNOS staining (P <.001).
This preliminary study has shown that iNOS expression correlates with severity of dysplasia, and it is also increased in those cases showing positive staining for p53. Further research is required to fully establish the relationship between iNOS and p53 in both dysplasia and oral squamous cell carcinoma.
一氧化氮（NO）已在多种人类癌症中得到研究，并且与肿瘤促进和抑制均有关联。野生型p53（而非突变型）蛋白对酶诱导型一氧化氮合酶（iNOS）的下调已在正常细胞和一些肿瘤中被证实，但这种关系在口腔上皮发育异常中尚未见报道。
使用抗iNOS和p53（克隆号DO-7）抗体对36例不同严重程度的口腔发育异常病例进行了免疫组织化学研究。统计分析显示，iNOS染色与发育异常分级之间存在显著相关性（P<.001），p53与iNOS染色之间也存在显著相关性（P<.001）。
这项初步研究表明，iNOS表达与发育异常的严重程度相关，并且在p53染色呈阳性的病例中也有所增加。需要进一步研究以充分确立iNOS与p53在发育异常和口腔鳞状细胞癌中的关系。
