Shishido R, Sano Y, Fukui K
Department of Plant Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
Mol Gen Genet. 2000 May;263(4):586-91. doi: 10.1007/s004380051205.
rDNA (18S-5.8S-25S rDNA) and 5S rDNA loci were visualized on the chromosomes of six species of the genus Oryza by fluorescence in situ hybridization (FISH) and the labeled rice chromosomes were identified based on their condensation patterns. As a result, the chromosomes harboring rDNA and/or 5S rDNA loci were determined in the complement for all the known rice genomes. Variation in the location of the rDNA loci indicated the transpositional nature of the rDNAs in the genus Oryza, as also suggested in Triticeae and Allium. Comparative analysis of the locations of rDNA loci among rice, maize and wheat revealed that variability in the physical location of the rDNA loci was characteristic of the genus Oryza and also of the genera of Gramineae. This variability in the location of the rDNA loci between evolutionarily related species is in sharp contrast to the conservation of the general order of genes in their genomes.
通过荧光原位杂交（FISH）在六种稻属物种的染色体上对核糖体DNA（18S - 5.8S - 25S核糖体DNA）和5S核糖体DNA位点进行了可视化分析，并根据其凝聚模式对标记的水稻染色体进行了鉴定。结果，在所有已知水稻基因组的染色体组中确定了含有核糖体DNA和/或5S核糖体DNA位点的染色体。核糖体DNA位点位置的变化表明了稻属中核糖体DNA的转座性质，这在小麦族和葱属中也有提及。对水稻、玉米和小麦中核糖体DNA位点位置的比较分析表明，核糖体DNA位点物理位置的变异性是稻属以及禾本科各属的特征。核糖体DNA位点在进化相关物种之间位置的这种变异性与它们基因组中基因的总体顺序的保守性形成了鲜明对比。
