Robertson L J, Gjerde B
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo.
J Food Prot. 2000 Jun;63(6):775-8. doi: 10.4315/0362-028x-63.6.775.
Published techniques for recovering parasites from fruit and vegetables are generally inadequate, with low and variable recovery efficiencies. Here we describe an improved methodology for analyzing fruit and vegetables for Giardia cysts, Cryptosporidium oocysts, and Ascaris eggs. The method includes washing procedures, sonication, and, for Giardia and Cryptosporidium, immunomagnetic separation. Identification is by immunofluorescence (Giardia and Cryptosporidium) or brightfield microscopy (Ascaris). Recovery efficiencies from lettuce, Chinese leaves, and strawberries were found to be approximately 67% for Giardia, 42% for Cryptosporidium, and 72% for Ascaris. Recovery efficiencies from bean sprouts tended to be more variable and lower. This could be due to material removed with the parasites during the washing procedures, which, in turn, appeared related to the age of the bean sprouts. It is therefore recommended that fruit and vegetables should be as fresh as possible when analyzed for parasites.
目前已发表的从水果和蔬菜中回收寄生虫的技术通常并不完善，回收效率较低且不稳定。在此，我们描述一种改进的方法，用于分析水果和蔬菜中的贾第虫包囊、隐孢子虫卵囊和蛔虫卵。该方法包括清洗程序、超声处理，对于贾第虫和隐孢子虫，还包括免疫磁珠分离。通过免疫荧光法（检测贾第虫和隐孢子虫）或明视野显微镜检查法（检测蛔虫）进行鉴定。从生菜、白菜和草莓中回收的效率分别为：贾第虫约67%，隐孢子虫约42%，蛔虫约72%。豆芽的回收效率往往更不稳定且较低。这可能是由于在清洗过程中寄生虫随一些物质被去除，而这又似乎与豆芽的存放时间有关。因此，建议在分析水果和蔬菜中的寄生虫时，应尽可能选择新鲜的样本。
