Di Benedetto M A, Di Piazza F, Oliveri R, Cerame G, Valenti R, Firenze A
Dipartimento di Igiene e Microbiologia, Università degli Studi di Palermo.
Ann Ig. 2006 Mar-Apr;18(2):101-7.
Techniques described for recovering Giardia and Cryptosporidium (oo)cysts from fruit and vegetables are generally inadequate and present variable recovery efficience and elevated costs. The aim of our study was to evaluate the recovery efficiency of a simple and economic technique to apply either to berry vegetables, like tomatoes and peppers, or to large leave vegetables, like lettuce and chicory. The method include contamination and further elution of the vegetables. Then sedimentation of (oo)cysts by centrifugation of the eluate of vegetables and their visualization by means of direct immunofluorescence. The higher recovery values for both protozoa were obtained in large leave vegetables with mean data above 70% for Giardia and 76% for Cryptosporidium, whereas the values observed in the berry vegetables were above 43% for Giardia and above 37% for Cryptosporidium on average.
描述的从水果和蔬菜中回收贾第虫和隐孢子虫（卵）囊的技术通常并不充分，回收率各异且成本高昂。我们研究的目的是评估一种简单且经济的技术的回收效率，该技术适用于浆果类蔬菜（如西红柿和辣椒）或大叶蔬菜（如生菜和菊苣）。该方法包括对蔬菜进行污染及进一步洗脱。然后通过对蔬菜洗脱液进行离心使（卵）囊沉淀，并通过直接免疫荧光对其进行可视化观察。在大叶蔬菜中，两种原生动物均获得了较高的回收率，贾第虫平均数据高于70%，隐孢子虫高于76%；而在浆果类蔬菜中，贾第虫平均回收率高于43%，隐孢子虫高于37%。
