Instituto de Ingeniería del Agua y Medio Ambiente, Universidad Politécnica de Valencia, Valencia, Spain.
J Food Prot. 2010 Jun;73(6):1138-40. doi: 10.4315/0362-028x-73.6.1138.
A field study in Valencia, Spain, was done to determine the occurrence of Giardia and Cryptosporidium on salad products that are frequently eaten raw, such as lettuces and Chinese cabbage, and in irrigation waters. Four water samples were taken weekly 1 month before harvesting the vegetables. All water samples were analyzed using techniques included in the U.S. Environmental Protection Agency Method 1623. Standard methods for detecting protozoan parasites on salad vegetables are not available. Published techniques for the isolation of parasites from vegetables generally have low and variable recovery efficiencies. In this study, vegetables were analyzed using a recently reported method for detection of Cryptosporidium oocysts and Giardia cysts on salad products. The waters tested were positive for both Cryptosporidium and Giardia. Of 19 salad products studied, we observed Cryptosporidium in 12 samples and Giardia in 10 samples. Recoveries of the Texas Red-stained Cryptosporidium and Giardia, which were used as internal controls, were 24.5% +/- 3.5% for Cryptosporidium and 16.7% +/- 8.1% for Giardia (n = 8). This study provides data on the occurrence of Cryptosporidium and Giardia in salad products in Spain. The method was useful in the detection of Cryptosporidium oocysts and Giardia cysts on the vegetables tested, and it provides a useful analytical tool for occurrence monitoring.
西班牙巴伦西亚的一项实地研究旨在确定经常生食的沙拉产品（如生菜和白菜）和灌溉水中是否存在贾第鞭毛虫和隐孢子虫。在收获蔬菜前 1 个月，每周采集 4 个水样。所有水样均采用美国环境保护署方法 1623 中包含的技术进行分析。目前尚无用于检测沙拉蔬菜中原生动物寄生虫的标准方法。已公布的从蔬菜中分离寄生虫的技术通常回收率低且变化较大。在这项研究中，使用最近报道的一种方法检测沙拉产品中的隐孢子虫囊和贾第虫包囊来分析蔬菜。测试的水均对隐孢子虫和贾第虫呈阳性。在所研究的 19 种沙拉产品中，我们在 12 个样品中观察到隐孢子虫，在 10 个样品中观察到贾第虫。用作内部对照的 Texas Red 染色隐孢子虫和贾第虫的回收率分别为 24.5% +/- 3.5%（n = 8）和 16.7% +/- 8.1%。本研究提供了西班牙沙拉产品中隐孢子虫和贾第虫发生情况的数据。该方法可用于检测测试蔬菜中的隐孢子虫卵囊和贾第虫包囊，为发生监测提供了有用的分析工具。
