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青蛙视网膜视杆细胞中视紫红质380的形成。

The formation of metarhodospin380 in the retinal rods of the frog.

作者信息

Baumann C

出版信息

J Physiol. 1976 Jul;259(2):357-66. doi: 10.1113/jphysiol.1976.sp011470.

Abstract
  1. The formation of metarhodopsin380 (metarhodopsin II) was studied in isolated frog retinas exposed to intense flashes of 120 mus duration. 2. A rapid increase in absorbance at 375 nm during the flash was followed by a slower absorbance increase in the subsequent dark period. The slower increase showed virtual completion after 5 ms. 3. The fast absorbance increase during the flash was due to the formation of metarhodopsin478. The rate of this reaction was dependent on the time course of the flash and on the decay rate of lumirhodopsin. 4. Kinetic analysis indicates that three consecutive reactions occur: the light-controlled formation of lumirhodopsin, its first-order decay to metarhodopsin478 and the conversion of metarhodopsin478 into metarhodopsin380. At 21 degrees C, the decay constants were 2 X 10(4) S-1 (lumirhodopsin) and 1 X 10(3) S-1 (metarhodopsin478), respectively.
摘要
  1. 在暴露于持续时间为120微秒的强烈闪光下的离体青蛙视网膜中,研究了视紫红质380(视紫红质II)的形成。2. 闪光期间375纳米处吸光度迅速增加,随后在随后的暗期吸光度缓慢增加。较慢的增加在5毫秒后几乎完成。3. 闪光期间吸光度的快速增加是由于视紫红质478的形成。该反应的速率取决于闪光的时间进程和发光视紫红质的衰减速率。4. 动力学分析表明发生了三个连续反应:光控形成发光视紫红质,其一级衰减为视紫红质478,以及视紫红质478转化为视紫红质380。在21摄氏度时,衰减常数分别为2×10⁴秒⁻¹(发光视紫红质)和1×10³秒⁻¹(视紫红质478)。

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本文引用的文献

1
TAUTOMERIC FORMS OF METARHODOPSIN.变视紫红质的互变异构形式
J Gen Physiol. 1963 Nov;47(2):215-40. doi: 10.1085/jgp.47.2.215.
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Effect of flash illumination on rhodopsin in solution.
Ann N Y Acad Sci. 1959 Nov 12;74(2):281-90. doi: 10.1111/j.1749-6632.1958.tb39551.x.
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The early phase of dark adaptation.
Vision Res. 1972 Jun;12(6):1083-93. doi: 10.1016/0042-6989(72)90099-5.
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The influence of lipids on dynamic properties of rhodopsin.
Exp Eye Res. 1974 Jan;18(1):69-75. doi: 10.1016/0014-4835(74)90044-x.

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