离体人视网膜中视紫红质漂白的动力学
Kinetics of rhodopsin bleaching in the isolated human retina.
作者信息
Baumann C, Bender S
出版信息
J Physiol. 1973 Dec;235(3):761-73.
Abstract
- Pieces of human retina were dissected from eyes enucleated because of malignant tumours. The isolated retinas were perfused by an ionic medium (36 degrees C) and investigated spectrophotometrically.2. Rhodopsin was identified on the basis of its difference spectrum. The maximum absorbance change on bleaching was about 0.1 (lambda = 500 nm).3. The process of bleaching was quantitatively analysed in terms of four slow reactions, viz. (a) conversion of metarhodopsin II into metarhodopsin III, (b) hydrolysis of metarhodopsin II into retinal and opsin, (c) decay of metarhodopsin III to retinal, and (d) reduction of retinal to retinol.4. First-order rate constants for the reactions in 3 were 9 x 10(-3) sec(-1) (a), 3 x 10(-3) sec(-1) (b), 4 x 10(-3) sec(-1) (c), and 3 x 10(-2) sec(-1) (d).5. With two simplified versions of the model summarized in 3 and 4, the description of experimental data was less accurate.
摘要
从因恶性肿瘤而摘除的眼睛中取出人视网膜组织块。将分离出的视网膜用离子介质（36摄氏度）灌注，并进行分光光度法研究。
根据视紫红质的差示光谱对其进行鉴定。漂白时最大吸光度变化约为0.1（波长=500纳米）。
根据四个缓慢反应对漂白过程进行了定量分析，即：（a）变视紫红质II转化为变视紫红质III，（b）变视紫红质II水解为视黄醛和视蛋白，（c）变视紫红质III衰变为视黄醛，（d）视黄醛还原为视黄醇。
3中反应的一级速率常数分别为9×10⁻³秒⁻¹（a）、3×10⁻³秒⁻¹（b）、4×10⁻³秒⁻¹（c）和3×10⁻²秒⁻¹（d）。
用3和4中总结模型的两个简化版本对实验数据的描述准确性较低。
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